2016 addgene Search Results


2016 addgene  (Addgene inc)
92
Addgene inc 2016 addgene
2016 Addgene, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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stargazin gfp lovpep sequence  (Addgene inc)
93
Addgene inc stargazin gfp lovpep sequence
Figure 5. Junction elongation induces Rho flares. (A) Graph showing that calcium flash (GCaMP6m) follows junction elongation (verted-to-vertex length) and precedes Rho-mediated junction contraction and stabilization of length. Time 0 s represents start of Rho flare. Shaded region represents SEM; n = 16 flares, 13 embryos, 10 experiments. (B) Schematic of mosaic injection and regional activation of Rho-mediated contractility using optogenetics. Top: Schematic showing the mosaic injection of optogenetic constructs (prGEF and <t>LOVpep)</t> injected into one cell, while active Rho probe is injected in both cells at two-cell stage. Bottom: Regional activation of RhoA using optogenetics in region of stimulation (shaded gray box) and quantification of junction length and frequency of Rho flares in region of observation (dotted blue box). (C) Cell view of an embryo mosaically-expressing prGEF (2xPDZ-YFP-LARG(DH), yellow) and active Rho probe (mCherry-2xrGBD, gray). (C9) Cell view of Rho probe in the embryo from C. Regional stimulation (white dashed box) induces Rho flares in areas neighboring the region of stimulation (yellow arrowheads). (D) Quantification of Rho flares in the area outside the region of stimulation shown in C9. Frequency of Rho flares occurring pre-stimulation (0–300 s) and post-stimulation (600–900 s) are matched for color and shape. Significance calculated using unpaired two-tailed t test; n = 6 embryos, 4 experiments. (E) Time-lapse montage of a junction highlighted in C9 (yellow box) expressing active Rho probe (mCherry- 2xrGBD). Following optogenetic stimulation (indicated by gray shaded box), an increase in junction length precedes Rho flare activation (yellow arrowhead). Junction length measured from vertex to vertex is indicated under each panel.
Stargazin Gfp Lovpep Sequence, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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brie in lenticrisprv2  (Addgene inc)
90
Addgene inc brie in lenticrisprv2
Figure 5. Junction elongation induces Rho flares. (A) Graph showing that calcium flash (GCaMP6m) follows junction elongation (verted-to-vertex length) and precedes Rho-mediated junction contraction and stabilization of length. Time 0 s represents start of Rho flare. Shaded region represents SEM; n = 16 flares, 13 embryos, 10 experiments. (B) Schematic of mosaic injection and regional activation of Rho-mediated contractility using optogenetics. Top: Schematic showing the mosaic injection of optogenetic constructs (prGEF and <t>LOVpep)</t> injected into one cell, while active Rho probe is injected in both cells at two-cell stage. Bottom: Regional activation of RhoA using optogenetics in region of stimulation (shaded gray box) and quantification of junction length and frequency of Rho flares in region of observation (dotted blue box). (C) Cell view of an embryo mosaically-expressing prGEF (2xPDZ-YFP-LARG(DH), yellow) and active Rho probe (mCherry-2xrGBD, gray). (C9) Cell view of Rho probe in the embryo from C. Regional stimulation (white dashed box) induces Rho flares in areas neighboring the region of stimulation (yellow arrowheads). (D) Quantification of Rho flares in the area outside the region of stimulation shown in C9. Frequency of Rho flares occurring pre-stimulation (0–300 s) and post-stimulation (600–900 s) are matched for color and shape. Significance calculated using unpaired two-tailed t test; n = 6 embryos, 4 experiments. (E) Time-lapse montage of a junction highlighted in C9 (yellow box) expressing active Rho probe (mCherry- 2xrGBD). Following optogenetic stimulation (indicated by gray shaded box), an increase in junction length precedes Rho flare activation (yellow arrowhead). Junction length measured from vertex to vertex is indicated under each panel.
Brie In Lenticrisprv2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sgrna targeting irf4  (Addgene inc)
90
Addgene inc sgrna targeting irf4
Figure 5. Junction elongation induces Rho flares. (A) Graph showing that calcium flash (GCaMP6m) follows junction elongation (verted-to-vertex length) and precedes Rho-mediated junction contraction and stabilization of length. Time 0 s represents start of Rho flare. Shaded region represents SEM; n = 16 flares, 13 embryos, 10 experiments. (B) Schematic of mosaic injection and regional activation of Rho-mediated contractility using optogenetics. Top: Schematic showing the mosaic injection of optogenetic constructs (prGEF and <t>LOVpep)</t> injected into one cell, while active Rho probe is injected in both cells at two-cell stage. Bottom: Regional activation of RhoA using optogenetics in region of stimulation (shaded gray box) and quantification of junction length and frequency of Rho flares in region of observation (dotted blue box). (C) Cell view of an embryo mosaically-expressing prGEF (2xPDZ-YFP-LARG(DH), yellow) and active Rho probe (mCherry-2xrGBD, gray). (C9) Cell view of Rho probe in the embryo from C. Regional stimulation (white dashed box) induces Rho flares in areas neighboring the region of stimulation (yellow arrowheads). (D) Quantification of Rho flares in the area outside the region of stimulation shown in C9. Frequency of Rho flares occurring pre-stimulation (0–300 s) and post-stimulation (600–900 s) are matched for color and shape. Significance calculated using unpaired two-tailed t test; n = 6 embryos, 4 experiments. (E) Time-lapse montage of a junction highlighted in C9 (yellow box) expressing active Rho probe (mCherry- 2xrGBD). Following optogenetic stimulation (indicated by gray shaded box), an increase in junction length precedes Rho flare activation (yellow arrowhead). Junction length measured from vertex to vertex is indicated under each panel.
Sgrna Targeting Irf4, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sgrna targeting pdcd1  (Addgene inc)
90
Addgene inc sgrna targeting pdcd1
Figure 5. Junction elongation induces Rho flares. (A) Graph showing that calcium flash (GCaMP6m) follows junction elongation (verted-to-vertex length) and precedes Rho-mediated junction contraction and stabilization of length. Time 0 s represents start of Rho flare. Shaded region represents SEM; n = 16 flares, 13 embryos, 10 experiments. (B) Schematic of mosaic injection and regional activation of Rho-mediated contractility using optogenetics. Top: Schematic showing the mosaic injection of optogenetic constructs (prGEF and <t>LOVpep)</t> injected into one cell, while active Rho probe is injected in both cells at two-cell stage. Bottom: Regional activation of RhoA using optogenetics in region of stimulation (shaded gray box) and quantification of junction length and frequency of Rho flares in region of observation (dotted blue box). (C) Cell view of an embryo mosaically-expressing prGEF (2xPDZ-YFP-LARG(DH), yellow) and active Rho probe (mCherry-2xrGBD, gray). (C9) Cell view of Rho probe in the embryo from C. Regional stimulation (white dashed box) induces Rho flares in areas neighboring the region of stimulation (yellow arrowheads). (D) Quantification of Rho flares in the area outside the region of stimulation shown in C9. Frequency of Rho flares occurring pre-stimulation (0–300 s) and post-stimulation (600–900 s) are matched for color and shape. Significance calculated using unpaired two-tailed t test; n = 6 embryos, 4 experiments. (E) Time-lapse montage of a junction highlighted in C9 (yellow box) expressing active Rho probe (mCherry- 2xrGBD). Following optogenetic stimulation (indicated by gray shaded box), an increase in junction length precedes Rho flare activation (yellow arrowhead). Junction length measured from vertex to vertex is indicated under each panel.
Sgrna Targeting Pdcd1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sgrna targeting 1110004e09rik  (Addgene inc)
90
Addgene inc sgrna targeting 1110004e09rik
Figure 5. Junction elongation induces Rho flares. (A) Graph showing that calcium flash (GCaMP6m) follows junction elongation (verted-to-vertex length) and precedes Rho-mediated junction contraction and stabilization of length. Time 0 s represents start of Rho flare. Shaded region represents SEM; n = 16 flares, 13 embryos, 10 experiments. (B) Schematic of mosaic injection and regional activation of Rho-mediated contractility using optogenetics. Top: Schematic showing the mosaic injection of optogenetic constructs (prGEF and <t>LOVpep)</t> injected into one cell, while active Rho probe is injected in both cells at two-cell stage. Bottom: Regional activation of RhoA using optogenetics in region of stimulation (shaded gray box) and quantification of junction length and frequency of Rho flares in region of observation (dotted blue box). (C) Cell view of an embryo mosaically-expressing prGEF (2xPDZ-YFP-LARG(DH), yellow) and active Rho probe (mCherry-2xrGBD, gray). (C9) Cell view of Rho probe in the embryo from C. Regional stimulation (white dashed box) induces Rho flares in areas neighboring the region of stimulation (yellow arrowheads). (D) Quantification of Rho flares in the area outside the region of stimulation shown in C9. Frequency of Rho flares occurring pre-stimulation (0–300 s) and post-stimulation (600–900 s) are matched for color and shape. Significance calculated using unpaired two-tailed t test; n = 6 embryos, 4 experiments. (E) Time-lapse montage of a junction highlighted in C9 (yellow box) expressing active Rho probe (mCherry- 2xrGBD). Following optogenetic stimulation (indicated by gray shaded box), an increase in junction length precedes Rho flare activation (yellow arrowhead). Junction length measured from vertex to vertex is indicated under each panel.
Sgrna Targeting 1110004e09rik, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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2016 • gpi 2xmcherry  (Addgene inc)
92
Addgene inc 2016 • gpi 2xmcherry
Figure 5. Junction elongation induces Rho flares. (A) Graph showing that calcium flash (GCaMP6m) follows junction elongation (verted-to-vertex length) and precedes Rho-mediated junction contraction and stabilization of length. Time 0 s represents start of Rho flare. Shaded region represents SEM; n = 16 flares, 13 embryos, 10 experiments. (B) Schematic of mosaic injection and regional activation of Rho-mediated contractility using optogenetics. Top: Schematic showing the mosaic injection of optogenetic constructs (prGEF and <t>LOVpep)</t> injected into one cell, while active Rho probe is injected in both cells at two-cell stage. Bottom: Regional activation of RhoA using optogenetics in region of stimulation (shaded gray box) and quantification of junction length and frequency of Rho flares in region of observation (dotted blue box). (C) Cell view of an embryo mosaically-expressing prGEF (2xPDZ-YFP-LARG(DH), yellow) and active Rho probe (mCherry-2xrGBD, gray). (C9) Cell view of Rho probe in the embryo from C. Regional stimulation (white dashed box) induces Rho flares in areas neighboring the region of stimulation (yellow arrowheads). (D) Quantification of Rho flares in the area outside the region of stimulation shown in C9. Frequency of Rho flares occurring pre-stimulation (0–300 s) and post-stimulation (600–900 s) are matched for color and shape. Significance calculated using unpaired two-tailed t test; n = 6 embryos, 4 experiments. (E) Time-lapse montage of a junction highlighted in C9 (yellow box) expressing active Rho probe (mCherry- 2xrGBD). Following optogenetic stimulation (indicated by gray shaded box), an increase in junction length precedes Rho flare activation (yellow arrowhead). Junction length measured from vertex to vertex is indicated under each panel.
2016 • Gpi 2xmcherry, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mito-gcamp2  (Addgene inc)
90
Addgene inc mito-gcamp2
Figure 5. Junction elongation induces Rho flares. (A) Graph showing that calcium flash (GCaMP6m) follows junction elongation (verted-to-vertex length) and precedes Rho-mediated junction contraction and stabilization of length. Time 0 s represents start of Rho flare. Shaded region represents SEM; n = 16 flares, 13 embryos, 10 experiments. (B) Schematic of mosaic injection and regional activation of Rho-mediated contractility using optogenetics. Top: Schematic showing the mosaic injection of optogenetic constructs (prGEF and <t>LOVpep)</t> injected into one cell, while active Rho probe is injected in both cells at two-cell stage. Bottom: Regional activation of RhoA using optogenetics in region of stimulation (shaded gray box) and quantification of junction length and frequency of Rho flares in region of observation (dotted blue box). (C) Cell view of an embryo mosaically-expressing prGEF (2xPDZ-YFP-LARG(DH), yellow) and active Rho probe (mCherry-2xrGBD, gray). (C9) Cell view of Rho probe in the embryo from C. Regional stimulation (white dashed box) induces Rho flares in areas neighboring the region of stimulation (yellow arrowheads). (D) Quantification of Rho flares in the area outside the region of stimulation shown in C9. Frequency of Rho flares occurring pre-stimulation (0–300 s) and post-stimulation (600–900 s) are matched for color and shape. Significance calculated using unpaired two-tailed t test; n = 6 embryos, 4 experiments. (E) Time-lapse montage of a junction highlighted in C9 (yellow box) expressing active Rho probe (mCherry- 2xrGBD). Following optogenetic stimulation (indicated by gray shaded box), an increase in junction length precedes Rho flare activation (yellow arrowhead). Junction length measured from vertex to vertex is indicated under each panel.
Mito Gcamp2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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addgene 65992  (Addgene inc)
93
Addgene inc addgene 65992
Figure 5. Junction elongation induces Rho flares. (A) Graph showing that calcium flash (GCaMP6m) follows junction elongation (verted-to-vertex length) and precedes Rho-mediated junction contraction and stabilization of length. Time 0 s represents start of Rho flare. Shaded region represents SEM; n = 16 flares, 13 embryos, 10 experiments. (B) Schematic of mosaic injection and regional activation of Rho-mediated contractility using optogenetics. Top: Schematic showing the mosaic injection of optogenetic constructs (prGEF and <t>LOVpep)</t> injected into one cell, while active Rho probe is injected in both cells at two-cell stage. Bottom: Regional activation of RhoA using optogenetics in region of stimulation (shaded gray box) and quantification of junction length and frequency of Rho flares in region of observation (dotted blue box). (C) Cell view of an embryo mosaically-expressing prGEF (2xPDZ-YFP-LARG(DH), yellow) and active Rho probe (mCherry-2xrGBD, gray). (C9) Cell view of Rho probe in the embryo from C. Regional stimulation (white dashed box) induces Rho flares in areas neighboring the region of stimulation (yellow arrowheads). (D) Quantification of Rho flares in the area outside the region of stimulation shown in C9. Frequency of Rho flares occurring pre-stimulation (0–300 s) and post-stimulation (600–900 s) are matched for color and shape. Significance calculated using unpaired two-tailed t test; n = 6 embryos, 4 experiments. (E) Time-lapse montage of a junction highlighted in C9 (yellow box) expressing active Rho probe (mCherry- 2xrGBD). Following optogenetic stimulation (indicated by gray shaded box), an increase in junction length precedes Rho flare activation (yellow arrowhead). Junction length measured from vertex to vertex is indicated under each panel.
Addgene 65992, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pklv2 ef1absd2acas9 w  (Addgene inc)
98
Addgene inc pklv2 ef1absd2acas9 w
Figure 5. Junction elongation induces Rho flares. (A) Graph showing that calcium flash (GCaMP6m) follows junction elongation (verted-to-vertex length) and precedes Rho-mediated junction contraction and stabilization of length. Time 0 s represents start of Rho flare. Shaded region represents SEM; n = 16 flares, 13 embryos, 10 experiments. (B) Schematic of mosaic injection and regional activation of Rho-mediated contractility using optogenetics. Top: Schematic showing the mosaic injection of optogenetic constructs (prGEF and <t>LOVpep)</t> injected into one cell, while active Rho probe is injected in both cells at two-cell stage. Bottom: Regional activation of RhoA using optogenetics in region of stimulation (shaded gray box) and quantification of junction length and frequency of Rho flares in region of observation (dotted blue box). (C) Cell view of an embryo mosaically-expressing prGEF (2xPDZ-YFP-LARG(DH), yellow) and active Rho probe (mCherry-2xrGBD, gray). (C9) Cell view of Rho probe in the embryo from C. Regional stimulation (white dashed box) induces Rho flares in areas neighboring the region of stimulation (yellow arrowheads). (D) Quantification of Rho flares in the area outside the region of stimulation shown in C9. Frequency of Rho flares occurring pre-stimulation (0–300 s) and post-stimulation (600–900 s) are matched for color and shape. Significance calculated using unpaired two-tailed t test; n = 6 embryos, 4 experiments. (E) Time-lapse montage of a junction highlighted in C9 (yellow box) expressing active Rho probe (mCherry- 2xrGBD). Following optogenetic stimulation (indicated by gray shaded box), an increase in junction length precedes Rho flare activation (yellow arrowhead). Junction length measured from vertex to vertex is indicated under each panel.
Pklv2 Ef1absd2acas9 W, supplied by Addgene inc, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pog44  (Thermo Fisher)
90
Thermo Fisher pog44
Figure 5. Junction elongation induces Rho flares. (A) Graph showing that calcium flash (GCaMP6m) follows junction elongation (verted-to-vertex length) and precedes Rho-mediated junction contraction and stabilization of length. Time 0 s represents start of Rho flare. Shaded region represents SEM; n = 16 flares, 13 embryos, 10 experiments. (B) Schematic of mosaic injection and regional activation of Rho-mediated contractility using optogenetics. Top: Schematic showing the mosaic injection of optogenetic constructs (prGEF and <t>LOVpep)</t> injected into one cell, while active Rho probe is injected in both cells at two-cell stage. Bottom: Regional activation of RhoA using optogenetics in region of stimulation (shaded gray box) and quantification of junction length and frequency of Rho flares in region of observation (dotted blue box). (C) Cell view of an embryo mosaically-expressing prGEF (2xPDZ-YFP-LARG(DH), yellow) and active Rho probe (mCherry-2xrGBD, gray). (C9) Cell view of Rho probe in the embryo from C. Regional stimulation (white dashed box) induces Rho flares in areas neighboring the region of stimulation (yellow arrowheads). (D) Quantification of Rho flares in the area outside the region of stimulation shown in C9. Frequency of Rho flares occurring pre-stimulation (0–300 s) and post-stimulation (600–900 s) are matched for color and shape. Significance calculated using unpaired two-tailed t test; n = 6 embryos, 4 experiments. (E) Time-lapse montage of a junction highlighted in C9 (yellow box) expressing active Rho probe (mCherry- 2xrGBD). Following optogenetic stimulation (indicated by gray shaded box), an increase in junction length precedes Rho flare activation (yellow arrowhead). Junction length measured from vertex to vertex is indicated under each panel.
Pog44, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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2016 pmxs  (Addgene inc)
96
Addgene inc 2016 pmxs
Figure 5. Junction elongation induces Rho flares. (A) Graph showing that calcium flash (GCaMP6m) follows junction elongation (verted-to-vertex length) and precedes Rho-mediated junction contraction and stabilization of length. Time 0 s represents start of Rho flare. Shaded region represents SEM; n = 16 flares, 13 embryos, 10 experiments. (B) Schematic of mosaic injection and regional activation of Rho-mediated contractility using optogenetics. Top: Schematic showing the mosaic injection of optogenetic constructs (prGEF and <t>LOVpep)</t> injected into one cell, while active Rho probe is injected in both cells at two-cell stage. Bottom: Regional activation of RhoA using optogenetics in region of stimulation (shaded gray box) and quantification of junction length and frequency of Rho flares in region of observation (dotted blue box). (C) Cell view of an embryo mosaically-expressing prGEF (2xPDZ-YFP-LARG(DH), yellow) and active Rho probe (mCherry-2xrGBD, gray). (C9) Cell view of Rho probe in the embryo from C. Regional stimulation (white dashed box) induces Rho flares in areas neighboring the region of stimulation (yellow arrowheads). (D) Quantification of Rho flares in the area outside the region of stimulation shown in C9. Frequency of Rho flares occurring pre-stimulation (0–300 s) and post-stimulation (600–900 s) are matched for color and shape. Significance calculated using unpaired two-tailed t test; n = 6 embryos, 4 experiments. (E) Time-lapse montage of a junction highlighted in C9 (yellow box) expressing active Rho probe (mCherry- 2xrGBD). Following optogenetic stimulation (indicated by gray shaded box), an increase in junction length precedes Rho flare activation (yellow arrowhead). Junction length measured from vertex to vertex is indicated under each panel.
2016 Pmxs, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Figure 5. Junction elongation induces Rho flares. (A) Graph showing that calcium flash (GCaMP6m) follows junction elongation (verted-to-vertex length) and precedes Rho-mediated junction contraction and stabilization of length. Time 0 s represents start of Rho flare. Shaded region represents SEM; n = 16 flares, 13 embryos, 10 experiments. (B) Schematic of mosaic injection and regional activation of Rho-mediated contractility using optogenetics. Top: Schematic showing the mosaic injection of optogenetic constructs (prGEF and LOVpep) injected into one cell, while active Rho probe is injected in both cells at two-cell stage. Bottom: Regional activation of RhoA using optogenetics in region of stimulation (shaded gray box) and quantification of junction length and frequency of Rho flares in region of observation (dotted blue box). (C) Cell view of an embryo mosaically-expressing prGEF (2xPDZ-YFP-LARG(DH), yellow) and active Rho probe (mCherry-2xrGBD, gray). (C9) Cell view of Rho probe in the embryo from C. Regional stimulation (white dashed box) induces Rho flares in areas neighboring the region of stimulation (yellow arrowheads). (D) Quantification of Rho flares in the area outside the region of stimulation shown in C9. Frequency of Rho flares occurring pre-stimulation (0–300 s) and post-stimulation (600–900 s) are matched for color and shape. Significance calculated using unpaired two-tailed t test; n = 6 embryos, 4 experiments. (E) Time-lapse montage of a junction highlighted in C9 (yellow box) expressing active Rho probe (mCherry- 2xrGBD). Following optogenetic stimulation (indicated by gray shaded box), an increase in junction length precedes Rho flare activation (yellow arrowhead). Junction length measured from vertex to vertex is indicated under each panel.

Journal: The Journal of cell biology

Article Title: Mechanosensitive calcium flashes promote sustained RhoA activation during tight junction remodeling.

doi: 10.1083/jcb.202105107

Figure Lengend Snippet: Figure 5. Junction elongation induces Rho flares. (A) Graph showing that calcium flash (GCaMP6m) follows junction elongation (verted-to-vertex length) and precedes Rho-mediated junction contraction and stabilization of length. Time 0 s represents start of Rho flare. Shaded region represents SEM; n = 16 flares, 13 embryos, 10 experiments. (B) Schematic of mosaic injection and regional activation of Rho-mediated contractility using optogenetics. Top: Schematic showing the mosaic injection of optogenetic constructs (prGEF and LOVpep) injected into one cell, while active Rho probe is injected in both cells at two-cell stage. Bottom: Regional activation of RhoA using optogenetics in region of stimulation (shaded gray box) and quantification of junction length and frequency of Rho flares in region of observation (dotted blue box). (C) Cell view of an embryo mosaically-expressing prGEF (2xPDZ-YFP-LARG(DH), yellow) and active Rho probe (mCherry-2xrGBD, gray). (C9) Cell view of Rho probe in the embryo from C. Regional stimulation (white dashed box) induces Rho flares in areas neighboring the region of stimulation (yellow arrowheads). (D) Quantification of Rho flares in the area outside the region of stimulation shown in C9. Frequency of Rho flares occurring pre-stimulation (0–300 s) and post-stimulation (600–900 s) are matched for color and shape. Significance calculated using unpaired two-tailed t test; n = 6 embryos, 4 experiments. (E) Time-lapse montage of a junction highlighted in C9 (yellow box) expressing active Rho probe (mCherry- 2xrGBD). Following optogenetic stimulation (indicated by gray shaded box), an increase in junction length precedes Rho flare activation (yellow arrowhead). Junction length measured from vertex to vertex is indicated under each panel.

Article Snippet: Stargazin-GFP-LOVpep (referred to as GFPLOVpep) was generated by PCR amplifying the Stargazin-GFP- LOVpep sequence (Addgene plasmid #80406; Wagner and Glotzer, 2016) and ligating into the ClaI site in pCS2+.

Techniques: Injection, Activation Assay, Optogenetics, Construct, Expressing, Two Tailed Test